The Rowdyruff Boys officially reappeared in the show, in the episode "The Boys are Back in Town", due partially to the requests by fans. The character HIM brought them back to life and added an anti-cootie vaccination to prevent the girls' kisses from having the prior deadly effect. The vaccination instead made the boys grow "bigger and more powerful." But unfortunately, the girls found this out much too late and made the boys grow many times thier previous size. The boys get their revenge on the girls, firstly by grossing them out in various ways. It has been said that girls can make boys uncomfortable by being affectionate towards them, alternatively, boys can make girls uncomfortable by doing gross things in front of them. Battle soon commences and the boys effortlessley defeat the girls. Soon after, Buttercup makes Butch bite his tongue and he almost cries in pain. He shrinks under his brothers' laughter and Blossom realises that when the boys' masculinity is threatened, they shrink in size. The girls use makeup, pull down their pants and baby them so the boys shrink down to smaller than the buckles of the girls' shoes. The boys cry in shame and embarrassment, but Him soon arrives to yell at the boys for not defeating the girls and soon puts them back to their nornal size.


The Rowdyruff Boys have different hairstyles and slightly different personalities (sexism, male chauvinism, and misogyny to some) from their first incarnation. Unlike their first episode, the boys are not destroyed, and become regularly appearing characters in the show.

In one episode entitled, "Bubble Boy", the Powerpuff Girls kidnap Boomer after he has been ordered by Brick to buy sweets. Bubbles disguises herself as Boomer wearing his clothes and copying his hairstyle. There are several moments when Bubbles is close to letting it slip to Brick and Butch, who are still unaware that the real Boomer is held hostage, who she really is. There is even a moment when Brick looks like he suspects that Boomer is really Bubbles when he notices her panic about a cockroach.

They later appear in their third episode The City of Clipsville, in which the girls have flashbacks to various things (most of which were new/unaired scenes), including going into the future and becoming teenagers. The Rowdyruff Boys are also teenagers in this, and despite them being known as "bad boys" (as the girls deem them), have dulled their nature and become more slacker-ish about it. Butch even drives an Escalade, which the ditzy teenage Buttercup confuses for an escalator. Despite this, the girls all seem to have crushes on their male counterparts, and the boys likewise appear to feel the same way.

In another episode, titled Custody Battle, Mojo Jojo and Him have a major argument over which is the Rowdyruff Boys' true father and more evil parent. The boys witness several of Mojo and Him's attempts to prove their evil. In some cases they are impressed, but at the end of the episode, obviously now fed up, the boys ultimately break up the fight by proclaiming that they only care about destroying the Powerpuff Girls, which brought tears of joy to the two villans.

The Rowdyruff Boys do not appear to consider other villains either comrades or competition. In the episode "Bubble Boy," they remorselessly take over Fuzzy Lumpkins' cabin, leaving him tied up in a corner, and in "Boys Toys," reject Princess Morebucks' offer of alliance on account of her being a girl. In "Custody Battle," they have little respect for either Him or Mojo Jojo, particularly Mojo.

Currently, the Boys are now starring in new stories written for the Cartoon Network Action Pack! comic book. In the Powerpuff Girls 10th anniversary, it is revealed that the three are ranked at number 2 of the worst villains; only behind Mojo Jojo (their creator). Also notable is that in episodes like "See Me, Feel Me Gnomey" and the Powerpuff Girls Rule Special, they are not among the amalgam of villains or the corresponding frenzy.