This is how I see the characters, my opinions so not everyone will agree with me. Will add more to the list if I think of them.
France and America- friends/could be lovers
France and England- frenemies/lovers
France and Italy- brothers
America and England- brothers/father and son
France and Canada- brothers/father and son
America and Canada- Twin brothers
America and Japan- friends/lovers
Italy and Germany- friends/lovers
Italy and Japan- friends/could be lovers
Italy and Romano- brothers
France and Spain- friends/could be lovers
France and Prussia- friends
Spain and Prussia- friends
Prussia and Germany-...
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