The show's ponies may be grouped by age and sex into colts (younger males), fillies (younger females), mares (older females), and stallions (older males). A pony's sex can be ascertained by the shape and size of their muzzle: stallions' muzzles are angular, squared off and larger, while mares' muzzles have a more rounded appearance and are much smaller than a stallion's. However, this is not the case with Alicorns. Stallions also have bigger and sometimes bulkier bodies than mares. Another way to determine a pony's sex is by their eyelashes and that mares' hooves are the same color as their coat, while some stallions' hooves are coatless, like Big McIntosh's, Shining Armor's, or Prince Blueblood's. Mares and fillies have eyelashes, while stallions and colts don't. However, baby ponies have eyelashes, with males having a single eyelash, and females having two. In Lauren Faust's head,[19] at least one[20] S1 pony character[21] is trans.[22]
Twilight Sparkle: The main protagonist. An Alicorn (originally unicorn) pony with a light violet body and an indigo mane and tail with pink and purple streaks. She studies extensively and has exceptional magical ability. She is now one of the princesses of Equestria. Her spirit represents the element of magic.